In LUAD, LUSC (Lung Squamous Cell Carcinoma), PRAD (Prostate Cancer), UCEC (Endometrioid Cancer), BLCA (Bladder Cancer), TGCT (Testicular Cancer), ESCA (Esophageal Cancer), LIHC, CESC, SARC (Sarcoma), BRCA, COAD, SKCM, CHOL (Bile Duct Cancer), KIRC, THCA (Thyroid Cancer), HNSC (Head and Neck Cancer), LGG, KICH (Kidney Chromophobe), and UVM, the expression of NCS1 showed a positive correlation with immune checkpoint genes, especially in TGCT, LIHC, BRCA, SKCM, COAD, KICH, and UVM. This evidence concerns the gene NCS1 and squamous cell lung carcinoma.