In LUAD, LUSC (Lung Squamous Cell Carcinoma), PRAD (Prostate Cancer), UCEC (Endometrioid Cancer), BLCA (Bladder Cancer), TGCT (Testicular Cancer), ESCA (Esophageal Cancer), LIHC, CESC, SARC (Sarcoma), BRCA, COAD, SKCM, CHOL (Bile Duct Cancer), KIRC, THCA (Thyroid Cancer), HNSC (Head and Neck Cancer), LGG, KICH (Kidney Chromophobe), and UVM, the expression of NCS1 showed a positive correlation with immune checkpoint genes, especially in TGCT, LIHC, BRCA, SKCM, COAD, KICH, and UVM. The gene discussed is NCS1; the disease is urinary bladder cancer.