DUX4 and facioscapulohumeral muscular dystrophy: First, they used a DUX4-activated reporter to isolate the DUX4-expressing subset of cells from a population of FSHD myoblasts, then, using RNA-seq, they compared the transcriptome of the DUX4-posivitive cells with DUX4-negative cells from the same population, and identified differentially regulated pathways.