Additionally, we used qRT-PCR to reanalyze the NF-κB p65 (RelA) and TNFα gene expression levels and transcripts by calculating the relative fold change in gene expression between a control sample (TANT) and experimental samples (samples from GBM patients) through the ΔΔCt normalizing method and discovered their higher levels. This evidence concerns the gene NFKB1 and glioblastoma.