ESR2 and breast adenocarcinoma: Therefore, to investigate whether the combined S30 and LGA1, denoted as S30+LGA1, could increase the estrogen level, the mRNA expression of the genes of estrogen receptors, ESR1 and ESR2, and an estrogen-related gene of pS2 in MCF-7 cells [35,36] from human breast adenocarcinoma cell line, were analyzed.