In our previous study [14], we found that gut colonization with fecal microbiome transplantation (FMT) completely prevented the prolongation of NTG-induced migraine-like pain in germ-free mice and that tumor necrosis factor alpha (TNFα) in the spinal trigeminal nucleus caudalis (Sp5C) may contribute to the gut microbiome-produced regulation of migraine-like pain. This evidence concerns the gene TNF and migraine disorder.