TMEM147 and hepatocellular carcinoma: To investigate the effect of TMEM147 on HCC, we constructed lentivirus vectors to upregulate and silence TMEM147 in Huh7 and HepG2 cells; western blotting and qRT-PCR were used to confirm the overexpression and knockdown efficiency of TMEM147 (Fig. S2a and S2b).