Subsequently, we once again employed IF staining again to determine whether OLR1 in podocytes and the results confirmed that OLR1 exhibited segmental expression along the podocyte membrane protein marker, Nephrin, while also co-localizing with the nuclear marker protein, WT1, in both DKD renal biopsies and the mouse model (Fig. 4C). This evidence concerns the gene NPHS1 and diabetic kidney disease.