In this paper, we used SH-SY5Y human neuroblastoma cells stably transfected with the human Swedish amyloid precursor protein (APP) mutation APP695 (APP695swe SH-SY5Y) as an in vitro model and Caenorhabditis elegans (C. elegans) as an in vivo model to systematically investigate the neuroprotective effects of liensinine and neferine. This evidence concerns the gene APP and neuroblastoma.