The regulatory network of TF-miRNA-mRNA and lncRNA-miRNA-mRNA was predicted and revealed that has-miR-3127-5p and has-miR-3184-5p might be the regulator of PRM2、FSCN3 and TEKT2 and play a critical role in azoospermia and TGCT. This evidence concerns the gene FSCN3 and Azoospermia.