Among them, miRNAs (has-miR-3127-5p and has-miR-3184-5p), lncRNAs (AL356488.2 and AL645608.3) and TFs (NANOG and HEY1) as the key regulatory factors of PRM2, FSCN3 and TEKT2, were predicted significantly associated with male infertility. The gene discussed is PRM2; the disease is male infertility.