To clarify the time sequence of the events in CKD mouse brain including increased insoluble tau, BBB disruption, and increased serum urea, male C57BL/6 mice were exposed to different allocation periods of a 0.20%-adenine diet (2 days, 8 days, and 15 days) (Supplementary Figure 4A). The gene discussed is MAPT; the disease is chronic kidney disease.