Next, in order to investigate the effect of Fn and H2S on the gene pathway of cancer cells, a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed for the HCT116 + Fn group and HCT116 group, then subsequently the HCT116 + Fn + Cys group and HCT116 group.a When HCT116 + Fn was compared with the HCT116 group (Fig. 4b top), in addition to the upregulation of mitophagy, a number of other abnormalities were noted. This evidence concerns the gene FN1 and cancer.