Due to the fact that integrins, which are a family of heterodimeric cell surface receptors expressed on a wide variety of tumors, are responsible for regulating crucial cellular functions such as angiogenesis, adhesion, migration, and invasion [31], in this study, the intratumoral accumulation of IRDye 800CW RGD could be detected and adequately quantified in both cancer cell lines, and there was greater signal intensity for the C6 line. This evidence concerns the gene CD177 and cancer.