To understand how Npc2c impinges on 20E signaling to control ISC activity, we assessed the expression of two known EcR targets: the transcription factor Broad (Br) and the nuclear receptor and PPARγ-homologue, Eip75B, upon progenitor-specific Npc2c silencing in the presence or absence of infection, with or without RH5849 supplementation. The gene discussed is PPARG; the disease is infection.