IL27 and chronic obstructive pulmonary disease: The 16HBE cells were treated with the pro-neutrophilic chemokines IL-8, GROα, and RANTES and with cytokines related to monocyte–macrophage activation typically expressed in the bronchi of COPD patients (MIP1α and IL-27) [24,25] (all at 10 and 100 ng/mL) for up to 24 h (Figure 3).