Comparing the results with our previous studies, it was observed that HSPB1 was characterized by an increased expression after exposure to hyperthermia in seven of ten analyzed cell lines (colorectal cancer, ovarian cancer, and melanoma), whereas increased DNAJC4 expression was only observed in five of ten cell lines (colorectal cancer and melanoma), which may indicate their important role in the process of stimulating rAAV/DJ transduction at hyperthermia. This evidence concerns the gene HSPB1 and melanoma.