Methyl donor feeding, containing folic acid, betaine, choline, and vitamin B12, is involved in the development of obesity and lipid metabolism in association with sterol regulatory element-binding protein 2 (Srebf2) and 1-acylglycerol-3-phosphate O, which modify mRNA expression, and positively affects DNA methylation patterns in the promoter regions of acyltransferase 3 (Agpat3) and estrogen receptor 1 (Esr1) genes, reversing liver fat accumulation in mice that are fed a diet high in fat and sugar [69]. This evidence concerns the gene ESR1 and obesity due to melanocortin 4 receptor deficiency.