Induced membrane vesicles were isolated from native and genetically modified M14 tumor cells as well as from MDA-MB 231 cells by treating the cells with cytochalasin B. It was shown that the size of isolated CIMVs from M14 averaged 240 nm (SD ± 70), from M14-GM-CSF averaged 250 nm (SD ± 50), and from MDA-MB 231 averaged 190 nm (SD ± 80) (Figure 2B). Here, CSF2 is linked to neoplasm.