Our findings show that 1) AAVolig001 has primary Schwann cell tropism via intrasciatic delivery in rats and 2) AAVolig001-PZ1shRNA-mediated selective RNAi silencing of Piezo1 in both mSCs and nmSCs alleviates the mechanical hypersensitization representing allodynia and hyperalgesia following peripheral nerve injury, while cold and heat hypersensitivity are not significantly reduced. This evidence concerns the gene PIEZO1 and peripheral nerve injury.