As observed upon removal of extracellular Ca2+, combined inhibition of NCX, NMDA receptors, and TRPV4 channels during chemical ischemia led to a peak EM of -68.7 ± 3.4 mV (n = 9/9/3, p = 1 × 10−4) and data were affiliated to a single cluster (Figure 7B). Here, TLX2 is linked to ischemia.