ESR1 and cancer: RNA-Seq analysis revealed that upregulated genes in MCF-7/CerS4 cells compared with control MCF-7 cells included well-known cancer-related transcription factors, such as ERG, MYC, ESR1, TP53, SP1, EP300, RAD21, NFKB1, SMAD3, and HDAC2 (Table 2).