Two genes, GOLM1 and LIMK1, whose mRNA levels were decreased (p < 0.001) with the increase of miR-27a-3p were further validated in both HEK-293T cells and in epileptic mice.<h4>Conclusions</h4>MiR-27a-3p exhibits potential as a diagnostic and therapeutic marker for epilepsy. This evidence concerns the gene GOLM1 and epilepsy.