TRERF1 and colon adenocarcinoma: Moreover, when SW480 cells (a human colon adenocarcinoma line) were engineered to overexpress of Scribble and then exposed to rapamycin (Rapa; 100 μM for 4 h, a condition known to induce autophagy40), the LC3II/LC3I ratio was elevated (compared to that in rapamycin-exposed control SW480 cells) (Supplementary Fig. 4C).