Using the linear combination of IGSF8 and ITGA5 on the ovarian cancer (n = 10) and healthy control samples (n = 20) on this specificity data set, we achieved a PPV of 15.3% with a sensitivity of 0.90 at specificity of 99.8%, confirming the reliability of our original marker set (Supplementary Table 3). This evidence concerns the gene IGSF8 and ovarian cancer.