The volcano plots illustrated the differentially expressed genes, and the results showed that ZFYVE28 expression was lower in the peripheral blood of the obese-IS patients but higher in MetS patients with insulin resistance (Fig. 1b, d), which was further verified in the remaining samples (87 controls vs. 87 obese-IS patients, 87 controls vs. 87 MetS patients) by RT-qPCR (Fig. 1c, e). This evidence concerns the gene ZFYVE28 and Insulin resistance.