ZFYVE28 and infection: To further investigate the effect of ZFYVE28 on insulin sensitivity, we generated a mouse model with liver-specific overexpression of Zfyve28 (replaced by Zfyve28-LOE in the subsequent narrative) by tail vein injection of AAV9-pTBG-Zfyve28, in which liver-specific infection was achieved by a combination of the serotype AAV9, a serotype that efficiently infected the liver of mice, and liver-specific promoter pTBG (Supplementary Fig. 6a).