To study the effects of VCP mutations on neuronal homeostasis, we generated highly enriched and previously functionally validated human cortical excitatory neurons27 from hiPSCs derived from three independent cell lines from healthy individuals (termed ‘control neurons’ hereafter) and three independent cell lines with autosomal-dominant mutations in VCP that are causal for FTD (termed ‘VCP mutant neurons’ hereafter) (see Supplementary Fig. 1A and B for details of the cell lines). Here, VCP is linked to frontotemporal dementia.