After the initial 6 weeks of HFD feeding, female HFD-fed mice weighed more than LFD-fed control mice, exhibited a minor yet significant increase in blood glucose, and showed unaltered plasma insulin levels, while HFD-fed male mice exhibited increased body weight and hyperinsulinemia but no changes in blood glucose (Figures S2A–C, S4A–C). This evidence concerns the gene INS and hyperinsulinism.