We evaluated the number of CD4+ and CD8 + TIL per mm2 tumor area in those animals that developed tumors (3 TOP2A vaccinated and 12 CpG control mice) and observed that TOP2A vaccination significantly increased CD4+ and CD8 + TIL over adjuvant controls (Fig. 1f, g; p < 0.01). This evidence concerns the gene CD4 and neoplasm.