Notably, the top two most frequent TCR clonotypes, CALGDPGNTRKLIF_CASSLGGTGQLYF and CAVYQGGRALIF_CASSQRGIWENTGQLYF, both showed high binding affinity to the TOP2A peptide KDIVALMVRRAYDIA (Fig. 5c), suggesting that this TOP2A peptide plays an important role in the TOP2A vaccine-induced anti-tumor T cell response. The gene discussed is TOP2A; the disease is neoplasm.