As shown in Fig. 3c and d, DCIS spheroids expressing wtp53 exhibited a reduced replication rate (judged by BrdU labeling in Fig. 3c) and mTORC1 activation (evaluated by phosphorylation of its target 4EBP1) when grown in low AA. The gene discussed is EIF4EBP1; the disease is ductal breast carcinoma in situ.