In this study, to reveal the different roles of DR4 and DR5 in gastric cancer, we aimed to investigate the expression, sublocalization, and the clinical prognosis of DR4 and DR5 in gastric cancer tissue, and further confirm the high nucleus expression of DR5 in gastric cancer cells can promote tumor cells migration, invasion, and distant metastasis in vitro and in vivo. This evidence concerns the gene TNFRSF10B and gastric cancer.