CSF2 and neoplasm: In both CSF and GM, our cultured GBM cells (10 patients with GBM; 37,466 viable single cells) were very similar to the single cell–type composition reported by Neftel et al. (34) for cells from primary GBM tumors (9 patients with GBM; 8043 viable single cells), suggesting that our low-passage patient-derived cell lines represent clinically relevant tumor profiles (Fig. 4, A to C).