We found that the heterozygote of g.-1026 C>A–NOS2 (rs2779249) polymorphism was associated with a reduced risk of urolithiasis development in the female subpopulation (Crude OR 0.353; 0.138–0.902 95% CI; p < 0.05), while in the male subpopulation, we did not observe this association. This evidence concerns the gene NOS2 and urolithiasis.