In three subcohorts defined within the All of Us Research Program, the proportion of males ages 55–69 without a history of prostate cancer who received at least one PSA test in the first two years following cohort enrollment was lower (Cohort A: 11.1% [95% CI 10.6–11.6%], Cohort B: 12.4% [95% CI 11.8–13.0%], Cohort C: 16.8% [95% CI 16.2–17.3%]) than the proportion of respondents in the 2020 BRFSS survey who self-reported receiving a PSA test in the past two years (35.2%, 95% CI 34.2–36.3%). Here, KLK3 is linked to Familial prostate cancer.