Among alterations affecting the genes TP53, RB1, and PTEN, 34 SMs in 26 patients and two CNVs in 2 patients were detected in progressive tumor tissues and ctDNA, 10 SMs in 9 patients and 24 CNVs in 18 patients were detected only in tumor tissues, and 8 SMs in 8 patients were detected only in ctDNA. The gene discussed is RB1; the disease is neoplasm.