Here, the GSEA analysis showed that various genes enriched with ac4C were primarily distributed in ten signaling pathways, in which the Vegf signaling pathway with the highest normalized enrichment score (NES) was reportedly related to nerve injury.[31] Moreover, GSEA analysis also confirmed that Vegfa may be a hub gene that was regulated by ac4C modification in the spinal dorsal horn in neuropathic pain. The gene discussed is VEGFA; the disease is injury.