These cold, altered excluded, altered immunosuppressive, and hot tumor subtypes are characterized by low CD3+/CD8+ at the tumor center and margin, high CD3+/CD8+ at the tumor margin but little at the center, low CD3+/CD8+ at the tumor center or margin, and high CD3+ and low CD8+ at both the tumor center and margin. The gene discussed is CD8A; the disease is neoplasm.