We observed a NS1 dosage-dependent host response for genes involved in the inflammatory response (IL-6, IFNB1) and interferon stimulation (IFIT1, MX1, ISG15), with the IAV-NS1-T infection generating an intermediate phenotype at both the transcript (Figures 3A,B) and at the protein levels (Figure 3C). This evidence concerns the gene ISG15 and infection.