We next implemented a univariate and lasso-based regression model as well as performed a multivariate cox regression analysis on our specified regulated genes that resulted in our six-risk associated genes: MDGA2, DLX1, DSC1, UGT2B4, GNLY, and GGT2. Strikingly, the association of these genes with BLCA has not been established. This evidence concerns the gene GNLY and bladder transitional cell carcinoma.