In addition, 6-shogaol has synergistic effect with PI3K inhibitor (LY294002) to promote apoptosis of tumor cells, while it has antagonism with PI3K agonist (IGF-1) to eliminate the induction of apoptosis, which further indicates that 6-shogaol is a potential inhibitor of PI3K/Akt/mTOR signaling pathway [13]. This evidence concerns the gene AKT1 and neoplasm.