ACE and injury: Nevertheless, Donnarumma et al. [45] confirmed that ZOFE-mediated cardioprotection in murine and swine models of myocardial ischemia/reperfusion injury was associated with an increase in H2S levels, and Bucci et al. [15] confirmed that the release of H2S from zofenopril, an active metabolite of S-zofenopril, represented an additional beneficial mechanism unrelated to ACE inhibition.