In addition, ROC analysis revealed that the four genes had higher AUC values for predicting the diagnosis of breast cancer (TBC1D24, AUC = 0.798; TRIM67, AUC = 0.711; ZDHHC9, AUC = 0.810; QRSL1, AUC = 0.801) (Supplementary Fig. 2A–D). This evidence concerns the gene TBC1D24 and breast cancer.