Disease recurrence or resistance may be attributed to suppression of CAR-T cells during prolonged exposure to antigens.42 During the “resting phase”, without contact with tumour cells, CD8+ CAR-T cells from resistant patients showed higher expression of activation/inhibitor-related genes, and exhibited a premature activation and differentiation state compared to DR patients (Fig. 4), which may be one of the reasons why CAR-T cells from resistant patients quickly became dysfunctional after stimulation with tumour cells in vivo. This evidence concerns the gene CD8A and neoplasm.