IFNG and neoplasm: Moreover, when T cells isolated from the spleens of the above treated 4T1 tumor-inoculated mice were subjected to restimulation of 4T1 cell lysates, ELISpot assay showed markedly higher frequency of IFN-γ-secreting cells among T cells of mice received DOX@3D-MPs treatment than other treatments (Fig. 4n, o).