Meanwhile, the amounts of proliferated CD8+Ki67+ T (Fig. 4e), and functional CD8+CD69+ T (Fig. 4f), CD8+IL-2+ T (Fig. 4g), CD8+IFN-γ+ T (Fig. 4h), CD8+TNF-α+ T (Fig. 4i) and CD8+GzmB+ T cells (Fig. 4j) were the highest in tumor tissues of mice received DOX@3D-MPs treatment, indicating that DOX@3D-MPs exhibited the strong ability to activate antitumor immunity. This evidence concerns the gene CD69 and neoplasm.