The HEK293T cell line has been widely used as the model cell in studies of plasmid transfection and exploration of interactions between proteins.[37, 38] To further explore how FOXA2 was involved in CRC, IP and mass spectrometry (MS) analysis were conducted to investigate FOXA2 binding proteins using HCT‐116 and HEK293T cells stably transfected with FOXA2 (Figure 9A,B). Here, FOXA2 is linked to colorectal carcinoma.