In this study, IHC results showed that 100% (11/11), 100% (11/11), 81.8% (9/11) and 90.9% (10/11) of HCC cases were positive for PPIH, Ki-67, HepPar-1 and glypican-3, respectively (Figure 3). This evidence concerns the gene GPC3 and hepatocellular carcinoma.