The results suggest that the pathogenic/likely pathogenic variants (p.Tyr189Cys, p.Arg153Cys, p.Cys144Arg) of NOTCH3 were more correlated with MA, while the benign variants (p.Ala202=, p.Thr101= and p.Ala202=) appeared to be more associated with different types of headaches, including MO, tension-type headaches, and other types of headaches (Fisher’s exact test, p = 0.0233). This evidence concerns the gene NOTCH3 and Headache.