By performing pathological and immunoblotting (IB) analyses, we detected the protein levels of seven major reported immunosuppressive molecules in the primary and liver metastatic MC38 tumor cells, including FGL1, VISTA, programmed death-ligand 1/2 (PD-L1/2), high mobility group Box 1 (HMGB1), nectin cell adhesion molecule 2 (Nectin2) and galectin-9 (Gal-9)10. Here, VSIR is linked to neoplasm.