Owing to the low incidence of liver metastasis in orthotopic mouse model of CRC17,18, we constructed the intraportal transplantation model to evaluate the effects of FGL1 on tumor burden using immunocompetent syngeneic mice (C57BL/6 J) and immunodeficient Rag1-/- mice, which lack mature B and T cells due to V(D)J recombination deficiency19. The gene discussed is RAG1; the disease is neoplasm.