In comparison with Ifng-expressing CD4+ T cells in control mice, the top 20 most highly upregulated transcripts in Ifng-expressing CD4+ T cells in CPI colitis, included chemokines (Ccl3, Ccl4, Ccl5, Ccl9), other cytokines (Il22, Il13, Il17f, Il10, Spp1), and cytotoxicity molecules (Gzma, Gzmb, Prf1, Nkg7) (Fig. 6c). Here, SPP1 is linked to isolated cleft palate.