PRF1 and isolated cleft palate: In comparison with Ifng-expressing CD4+ T cells in control mice, the top 20 most highly upregulated transcripts in Ifng-expressing CD4+ T cells in CPI colitis, included chemokines (Ccl3, Ccl4, Ccl5, Ccl9), other cytokines (Il22, Il13, Il17f, Il10, Spp1), and cytotoxicity molecules (Gzma, Gzmb, Prf1, Nkg7) (Fig. 6c).