Interestingly, Sirt6−/+ infected macrophages treated with G9a inhibitor (BIX-01294) showed further decrease in cholesterol accumulation upon H37Rv infection, indicating a collegial role of G9a and SIRT6 in driving Mtb H37Rv infection-induced cholesterol accumulation (S2K and S2L Fig). The gene discussed is SIRT6; the disease is infection.