Several DEG that appeared commonly modulated by ZEB1 in KRAS- and BRAF-mutant CRC cells in the RNA-Seq were validated by quantitative real-time PCR (qRT-PCR); for instance, the downregulation of ZEB1, KRAS, or BRAF reduced mRNA levels of KLK10, DHRS2, PRDX3, and BABAM1, whereas it upregulated FAM3C mRNA (Figure 6E). This evidence concerns the gene KRAS and colorectal carcinoma.