LS174T and RKO CRC cells were transiently transfected with a control siRNA or a specific siRNA against ZEB1 (28) to generate LS174TCTL, RKOCTL, LS174TZEB1KD (where KD refers to knockdown), and RKOZEB1KD transgenic cell lines. This evidence concerns the gene ZEB1 and colorectal carcinoma.