Therefore, this study aimed to evaluate the possible roles of ACSS2 in kidney injury in DN by using kidney tissues from biopsied patients with DN, a podocyte cell culture model, and diabetic mice with an intragastric injection of a specific ACSS2 inhibitor, streptozotocin-treated ACSS2-knockout (ACSS2-KO) mice. Here, ACSS2 is linked to liver dysplastic nodule.